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primary antibodies col ii  (Boster Bio)


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    Boster Bio primary antibodies col ii
    Primary Antibodies Col Ii, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies col ii/product/Boster Bio
    Average 95 stars, based on 118 article reviews
    primary antibodies col ii - by Bioz Stars, 2026-05
    95/100 stars

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    In vitro chondrogenic assays. (a) Alcian blue staining of rBMSC cultured SF-PFS-KGN scaffolds after 14 days of culture in chondrogenic medium (scale bar = 3 mm). (b) Quantification of Alcian blue staining by measuring the absorbance of the eluent at 630 nm. (c) RT-qPCR results of mRNA expression of <t>COL</t> Ⅱ, ACAN, and Sox9 in rBMSC cultured scaffolds after 3, 7, and 14 days. (d) H&E and <t>COL</t> <t>II</t> staining of rBMSC cultured scaffolds after 14 days (scale bars = 1000 μm (15x); 50 μm (300x)). Data are presented as mean ± SD, n = 3. ## p < 0.01, ### p < 0.001. Compared with the pristine SF group: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    In vitro chondrogenic assays. (a) Alcian blue staining of rBMSC cultured SF-PFS-KGN scaffolds after 14 days of culture in chondrogenic medium (scale bar = 3 mm). (b) Quantification of Alcian blue staining by measuring the absorbance of the eluent at 630 nm. (c) RT-qPCR results of mRNA expression of <t>COL</t> Ⅱ, ACAN, and Sox9 in rBMSC cultured scaffolds after 3, 7, and 14 days. (d) H&E and <t>COL</t> <t>II</t> staining of rBMSC cultured scaffolds after 14 days (scale bars = 1000 μm (15x); 50 μm (300x)). Data are presented as mean ± SD, n = 3. ## p < 0.01, ### p < 0.001. Compared with the pristine SF group: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    In vitro chondrogenic assays. (a) Alcian blue staining of rBMSC cultured SF-PFS-KGN scaffolds after 14 days of culture in chondrogenic medium (scale bar = 3 mm). (b) Quantification of Alcian blue staining by measuring the absorbance of the eluent at 630 nm. (c) RT-qPCR results of mRNA expression of COL Ⅱ, ACAN, and Sox9 in rBMSC cultured scaffolds after 3, 7, and 14 days. (d) H&E and COL II staining of rBMSC cultured scaffolds after 14 days (scale bars = 1000 μm (15x); 50 μm (300x)). Data are presented as mean ± SD, n = 3. ## p < 0.01, ### p < 0.001. Compared with the pristine SF group: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: A sequential drug delivery system based on silk fibroin scaffold for effective cartilage repair

    doi: 10.1016/j.bioactmat.2025.03.005

    Figure Lengend Snippet: In vitro chondrogenic assays. (a) Alcian blue staining of rBMSC cultured SF-PFS-KGN scaffolds after 14 days of culture in chondrogenic medium (scale bar = 3 mm). (b) Quantification of Alcian blue staining by measuring the absorbance of the eluent at 630 nm. (c) RT-qPCR results of mRNA expression of COL Ⅱ, ACAN, and Sox9 in rBMSC cultured scaffolds after 3, 7, and 14 days. (d) H&E and COL II staining of rBMSC cultured scaffolds after 14 days (scale bars = 1000 μm (15x); 50 μm (300x)). Data are presented as mean ± SD, n = 3. ## p < 0.01, ### p < 0.001. Compared with the pristine SF group: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: The slices were incubated with the primary antibody against COL II (Servicebio) overnight at 4 °C in a wet box.

    Techniques: In Vitro, Staining, Cell Culture, Quantitative RT-PCR, Expressing